Journal: Science Advances

Article Title: Growth productivity as a determinant of the inoculum effect for bactericidal antibiotics

doi: 10.1126/sciadv.add0924

Figure Lengend Snippet: ( A ) ΔMIC kan as a function of % glucose y for A. faecalis , P. aeruginosa , and E. coli (from Fig. 2B ). SD from ≥3 biological replicates. For (A) and (B), SD from ≥4 different percentages of casamino acids per percentage of glucose. Raw data in fig. S10. Average residuals for growth curve fitting in table S3. ( B ) ΔMIC kan as a function of growth productivity. *ΔMIC kan not greater than zero (* P = 0.09, ** P = 0.20, one-tailed t tests). P and R 2 values on the figure from a linear regression. Shading denotes 95% confidence interval. Trends were significant using a Deming regression (table S10) and a WLS regression (table S11). Error bars: x axis = standard error from linear regression used to determine growth productivity, y axis = SD. Raw growth productivity data in fig. S10A. ( C ) ΔMIC kan as function of growth productivity for multiple bacterial species. Glucose = 0.04%. AB = A. baumannii , EC = E. coli , KP = K. pneumoniae , AF = A. faecalis , and PA = P. aeruginosa . We did not find a significant linear correlation between ΔMIC kan and [ATP] or maximum growth rate for P. aeruginosa and A. faecalis . Error bars: x axis = standard error from linear regression used to determine growth productivity, y axis = SD. We did not find a significant linear correlation between ΔMIC kan and [ATP] or maximum growth rate when all species were considered (glucose = 0.04%; fig. S10 and SM Results).

Article Snippet: P. aeruginosa strain PA14, K. pneumoniae strain (Carolina Biological, Burlington, NC), A. baumannii strain 5-109 (Carolina Biological), and A. faecalis (Carolina Biological) were used where indicated.

Techniques: One-tailed Test

Journal: BioMed Research International

Article Title: A Novel Microbisporicin Producer Identified by Early Dereplication during Lantibiotic Screening

doi: 10.1155/2015/419383

Figure Lengend Snippet: Antimicrobial activity of the screening extract from F31/11 broth measured as an endpoint in microdilution method, that is, the highest dilution that inhibits 80% of test strain growth.

Article Snippet: S. aureus Smith ATCC19636 (L819), Streptococcus pyogenes C203 ATCC12384 (L49), and other clinical isolates ( S. aureus L1400, Enterococcus faecalis L559, Enterococcus faecalis Van A L560, Escherichia coli SKF12140 L47, and Candida albicans SKF2270 L145) were maintained in the Fondazione Istituto Insubrico Ricerca per la Vita (F.I.I.R.V.) culture collection (L collection) at Gerenzano, Italy.

Techniques: Activity Assay